Table 1.
Specific primers for flavonoid biosynthetic genes in I. tinctoria.
| Gene names | Primer sequences (5′ to 3′) |
|---|---|
| PALa | Forward: TCACTCCTTCACTCCCTCTC |
| Reverse: TGATTCCCGCCATCTTGAAG | |
| C4Hb | Forward: AATCGACACGGTTCTTGGAC |
| Reverse: GCTTAGCGTCGTTGAGGTTC | |
| 4CLc | Forward: TTGCCGATGTTCCACATCTA |
| Reverse: GAGCAGCACCAGATTTCACA | |
| CHSd | Forward: GGGATAAGCGACTGGAACTC |
| Reverse: GAAACCAAACAAGACACCCC | |
| CHIe | Forward: CTTCCATCCTCTTCGCTCTC |
| Reverse: CAAGACTCAACCTAGCTCCAG | |
| F3′Hf | Forward: TCAAACACGTTGCACCAAAT |
| Reverse: GACTGCTTCTCCATCCAAGC | |
| FSg | Forward: CCACCTCATATCAGCGATCAAG |
| Reverse: GATTACAGGGAGGTGAACGAG | |
| Ubiquntin | Forward: ACCCTCACGGGGAAGACCATC |
| Reverse: ACCACGGAGACGGAGGACAAG |
a
PAL is expressed as phenylalanine ammonia lyase gene.
b
C4H is expressed as cinnamate-4-hydroxylase gene.
c
4CL is expressed as 4-coumarate coenzyme A ligase gene.
d
CHS is expressed as chalcone synthase gene.
e
CHI is expressed as chalcone isomerase gene.
f
F3′H is expressed as flavonoid 3′-hydroxylase gene.
g
FS is expressed as flavonol synthase gene.