Table 1.
Surface display of antigenic epitopes and mimotopes on filamentous phage
Library | Screened against | Results | Refs |
---|---|---|---|
12mer RPL displayed on pIII | Polyclonal sera specific for Burkholderia pseudomallei protease | Identification of linear and discontinuous protease epitopes of B. pseudomallei | [49] |
7mer RPLs displayed on pIII | Purified anti-Mycoplasma hyopneumoniae IgG | Identification of M. hyopneumoniae antigens. Phage clones identified by screening induced immune responses in murine model | [50] |
12mer RPL displayed on pIII | Monoclonal IgA1 specific for the capsule of Streptococcus pneumoniae | Identification of mimotopes of the capsular polysaccharide of type 8 S. pneumoniae. When conjugated to tetanus toxoid, mimotopes induced a type 8 capsular-polysaccharide-specific antibody response in mice | [51] |
7mer RPL displayed on pIII | Sera from swine infected with Nipah virus | Identification of several putative epitopes within the nucleocapsid protein of Nipah virus | [52] |
12mer RPL displayed on pIII | Polyclonal IgG specific for neutral polysaccharides of Mycoplasma tuberculosis | Isolation of phage clones that were antigenic mimotopes of B-cell epitopes of M. tuberculosis sugars. One clone invoked immune responses in rabbits | [53] |