Table 2.
Freeze–thaw, short-term and long-term stability of luteolin, apigenin, chrysoeriol and diosmetin in rat plasma. Data was expressed as remaining (%) of initial determined.
| Analyte | (μg/ml) | Freeze–thaw | 25 °C(4 h) | − 80 °C |
|
|---|---|---|---|---|---|
| 3 days | 7 days | ||||
| Luteolin | 0.05000 | 94.6 | 98.8 | 101.5 | 95.5 |
| 1.000 | 97.8 | 101.5 | 104.6 | 96.1 | |
| 5.000 | 98.9 | 97.4 | 93.6 | 94.5 | |
| Apigenin | 0.05000 | 93.5 | 95.2 | 100.6 | 91.7 |
| 1.000 | 95.1 | 101.5 | 104.2 | 91.2 | |
| 5.000 | 99.1 | 99.5 | 96.4 | 94.2 | |
| Chrysoeriol | 0.05000 | 94.3 | 94.8 | 96.4 | 93.6 |
| 1.000 | 95.6 | 100.6 | 98.6 | 92.5 | |
| 5.000 | 99.5 | 97.4 | 96.2 | 94.5 | |
| Diosmetin | 0.05000 | 100.0 | 95.5 | 99.8 | 90.5 |
| 1.000 | 95.5 | 100.1 | 100.9 | 94.1 | |
| 5.000 | 99.1 | 100.2 | 95.1 | 93.2 | |