Skip to main content
. 2016 Nov 23;90:187–194. doi: 10.1016/j.bios.2016.11.051

Fig. 3.

Fig. 3.

Comparison of limit of detection with iROAD assay and conventional method using T7-in vitro transcribed IFN-B RNA. (A) Resonance wavelength shift in iROAD assay. The colors represent the amount of the target: black (2.5×105 copies/reaction), gray with darker 50% (2.5×104 copies/reaction), gray with darker 35% (2.5×103 copies/mL), gray with darker 25% (2.5×102 copies/mL), gray with darker 15% (2.5×101 copies/reaction), and black dot (negative). (B) Linear relationship between wavelength by iROAD assay and the concentration of target in 20 min. Error bars indicate standard deviation from the mean, based on at least 3 independent experiments. (C) Linear relationship between the concentration of target and Ct value of fluorescence signal by real-time RT-PCR. 1: 2.5×109 copies/reaction, 2: 2.5×108 copies/reaction, 3: 2.5×107 copies/reaction, 4: 2.5×106 copies/reaction, 5: 2.5×105 copies/reaction, 6: 2.5×104 copies/reaction, 7: 2.5×103 copies/reaction, 8: 2.5×102 copies/reaction and 9: negative control..