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. 2015 Apr 30;33(26):2955–2962. doi: 10.1016/j.vaccine.2015.04.071

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© 2015

Since January 2020 Elsevier has created a COVID-19 resource centre with free information in English and Mandarin on the novel coronavirus COVID-19. The COVID-19 resource centre is hosted on Elsevier Connect, the company's public news and information website. Elsevier hereby grants permission to make all its COVID-19-related research that is available on the COVID-19 resource centre - including this research content - immediately available in PubMed Central and other publicly funded repositories, such as the WHO COVID database with rights for unrestricted research re-use and analyses in any form or by any means with acknowledgement of the original source. These permissions are granted for free by Elsevier for as long as the COVID-19 resource centre remains active.

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Fig. 1 — Schematic representation of the three plasmid platform used to produce pseudovirus. (1) The structural gag and pol enzymatic proteins are expressed from a single plasmid lacking a packaging signal. (2) The envelope (env) gene is expressed from a plasmid with a promoter (PRO) specific to the envelope. (3) The reporter gene is expressed from a plasmid incorporating a packaging signal (Ψ) and flanking long tandem repeats (LTR), along with an upstream promoter.