Figure 1.

SN domain of Tudor‐SN protein interacts with G3BP protein in vitro. (A) The schematic structure of Tudor‐SN protein. (B) The loading control of GST, GST‐SN, GST‐TSN and GST‐TD fusion proteins for (C) and (D), are visualized by Coomassie blue. (C) COS‐7 cells were transfected with GFP‐G3BP. After 36 h, the total cell lysate (TCL) were collected and incubated with beads‐bound GST, or different GST fusion proteins. The interacted proteins were subjected to SDS–PAGE and analyzed by blotting with anti‐GFP antibody. Twenty percent of TCL was included as input. (D) G3BP was ³⁵S‐labeled by in vitro translation and incubated with beads loaded with different GST fusion proteins or GST. The bound proteins were subjected to SDS–PAGE and visualized by autoradiography. Twenty percent of the in vitro‐translated protein was included as input.