Figure 4.

Effects of Cs₂K₄Na[SiW₉Nb₃O₄₀]·H₂O or adefovir dipivoxil on intracellular HBV DNA replication (A), intracellular HBcAg and HBsAg; (B) and intracellular mRNA; (c) in HepG2.2.15 cells. Cells were treated with compound for 4 days. Data are expressed as mean ± SD (n = 4). 1:1 μmol/L, 10:10 μmol/L, 100:100 μmol/L, NC: negative control, ADV: adefovir dipivoxil, X: Cs₂K₄Na[SiW₉Nb₃O₄₀]·H₂O (A) Intracellular HBV DNA replication was determined by Southern blot analysis. RC: Relaxed circular DNA; SS: Single stranded DNA. HepG2: HepG2 cell was used as control, which replaced HepG2.2.15 cell; (B) Intracellular HBcAg and HBsAg was determined by Western blot analysis; (C) Intracellular mRNA was determined by RT-PCR. Relative amounts of intracellular mRNA are presented as%, as compared to levels detected in non-treated controls (NC). ^∗P <0.05 versus the corresponding non-treated controls; ^#P <0.05 versus the corresponding positive controls at the same dose.