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. 2017 Feb 16;92:234–240. doi: 10.1016/j.bios.2017.02.023

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© 2017 Elsevier B.V. All rights reserved.

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Fig. 2 — Characterization of influenza A virus subtype H1N1. (A) Determination of HA titer by hemagglutination assay. A 16- to 2048-fold dilution series (2⁻⁴ to 2⁻¹¹) of virus solution was prepared using 0.01% gelatin DPBS solution. Then, 50 μL of each dilution was added to a 96-well round-bottom plate. Fifty microliters of 0.5% chicken red blood cell suspension was mixed with each virus solution (n=3). Top panel: 96-well plate just after mixing. Lower panel: 96-well plate after 30 min. The blood cells were precipitated from 1/512 dilution of virus solution, giving an HA titer of the original virus of 256. (B) The size distribution, mean size of particle diameter and zeta potential of virus (32 HAU).