Table 1.
Primers
| Set | Amplicon | Influenza A primers | Sequence (5′–3′) |
|---|---|---|---|
| 1 | 93 bp | FluA_Universal_1 F | AGCAAAAGCAGGTAGATRTT |
| FluA_Universal_1 R | TCGGCTTTGAGGGG | ||
| 2 | 239 bp | FluA_Universal_2 F | GACCIATCCTGTCACCTCTGAC |
| FluA_Universal_2 R | CATICAACTGGCIAGIGCAC | ||
| 2.5 | 239 bp | FluA_Universal_2.5 Fa | GACCRATYCTGTCACCTCTGAC |
| FluA_ Universal_2.5 R | CATYCAACTGGCAAGTGCAC | ||
| 3 | 647 bp | FMV_A (not H3N2) F | TACGTTCTCTCTATCATCCCG |
| FMV_A (not H3N2) R | GAGCTAGGATGAGTCCCAATAAC | ||
| 4 | 264 bp | Circ_H3N2 (not Vaccine) F | CTGTAACCACTGAAGTGGCA |
| Circ_H3N2 (not Vaccine) R | AGCTAGGATGAGTCCCAATGG | ||
| 5 | 264 bp | Circ_H1N1 (not Vaccine) Fb | CTGTRACCACCGAATCAGCA |
| 6 | 264 bp | Circ_H1N2 (not Vaccine) F | CTGTAACCACCGAAGTGGCA |
| Circ_H1N2 (not Vaccine) R | AACTAGGATGAGTCCCAACGG | ||
| Set | Amplicon | Influenza B primers | Sequence (5′–3′) |
| 7 | 91 bp | FluB_Universal F | GGATTAAACAAAAGCAAGCCTTCCT |
| FluB_Universal R | CAAGCTTCAAAGGTGTTTTC | ||
| 8 | 472 bp | FMV_B F | GCCTACCTGCTTTCACTA |
| FMV_B R | CTCTGCTCTGCTATGAGCTCTT | ||
| 9 | 472 bp | Circ_B F | GCCTACCTGCTTTCATTG |
| Circ_B R | CTCTGCTATGAGCCCTG | ||
All primers were developed by NHRC except the FluA_Universal_1 primers (developed by Luke Daum at Brooks Air Force Base, San Antonio, TX).
a
FluA_Universal_2.5 (set 2.5) is a current, drift-updated version of the FluA_Universal_2 primers. All FluA_Universal primers have been validated against culture results on more than 100 samples at NHRC and serve (or served) as part of our CAP-certified diagnostic RT-PCR for influenza A.
b
Circ_H1N1 F uses Circ_H3N2 R as a reverse primer.