Table 3.
Cycling conditions
| PCR step | Cycles | Flu_A_Universal_1 | Flu_A_Universal_2, 2.5 | Circ_H1N1 | FMV_A and B Circ_H3N2, H1N2, B |
|---|---|---|---|---|---|
| RT | 1 | 45:00 (50 °C) | 45:00 (50 °C) | 45:00 (50 °C) | 45:00 (50 °C) |
| Activation | 1 | 15:00 (95 °C) | 15:00 (95 °C) | 15:00 (95 °C) | 15:00 (95 °C) |
| Denaturation | 40a | 0:30 (94 °C) | 0:30 (94 °C) | 0:30 (94 °C) | 0:30 (94 °C) |
| Annealing | 1:00 (50 °C) | 1:00 (58 °C) | 1:30 (58 °C) | 1:30 (55 °C) | |
| Extension | 1:00 (72 °C) | 1:00 (72 °C) | 2:00 (72 °C) | 2:00 (72 °C) |
All conditions are shown as time (temperature), with time shown as minutes:seconds.
a
FluA_Universal primer sets are run for 35 cycles.