Table 1.
Primers and probe for HRV qPCR assay.
| Name | Sequence (5′ → 3′)a | Positionb |
|---|---|---|
| RAF, forwardc | GACAGTGTTCYAGCCTGCG | 342–360 |
| RBF, forwardc | RACHGTGTCYYAGCCTGCG | 342–360 |
| LR, reversed | GAAACACGGACACCCAAAGTA | 556–536 |
| Rhino-probed, e | TCCTCCGGCCCCTGAATGYGGC | 437–458 |
a
R = dA or dG; H = dA, dC, or dT; Y = dC or dT.
b
The nucleotide numbering is based on that of the HRV 60 (1473) sequence (accession no. FJ445143.1).
c
Primers modified from Lu et al. (2008).
d
Adapted from Lu et al. (2008).
e
The probe was 5′-end labeled with 6-carboxyfluorescein and 3′-end labeled with Black Hole Quencher-1 (Biosearch Technologies).