Table 3.
Results of typing of field pestivirus strains by two nPCR assays.
| Method | Reference | BVDV-1 | BVDV-2 | ‘Hobi’-like | BDV | Not typed |
|---|---|---|---|---|---|---|
| nPCR (Erns) | Ref. [35] | 80 | 20 | NA | 0 | 0 |
| nPCR (5’ UTR, Npro, C) | This study | 80 | 15 | 3 | NA | 2b |
| Sequence analysisa | NA | ND | ND | 3 | 2b | NA |
NA, not applicable; ND, not done.
a
Sequence analysis was carried out only on the five pestivirus strains that were differently typed by the two nPCR assays.
b
Strains detected in tissue samples from dead kids, that were erroneously typed as BVDV-2 by the Erns nPCR and found to be true BDVs by sequence analysis.