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. 2012 Jul 20;53(3):162–167. doi: 10.1016/j.micpath.2012.07.002

Fig. 1.

Fig. 1

Enumeration of SFCs in a 24-h direct ELISPOT assay performed with freshly isolated PBMCs from HLA-A24+ TB donors, HLA-A24− TB donors, or HLA-A2 TB donors. (A) Results are means ± SD from three separate experiments. The spots are a measure of IFN-γ-producing cells from PBMCs stimulated with various candidate peptides. (B) Results are from one representative experiment. 5 μg/ml PHA and TB-stimulants were used as positive controls. No peptide was added in the negative control.