Fig. 3.

In vitro refolding of the HLA-A24 heavy chain and β2m molecules with candidate peptides of ESAT-6 and CFP10 proteins. The refolded complexes were tested by fast protein liquid chromatography Superdex G75 gel filtration. The positive control (PC) peptide was a HLA-A24-restricted severe acute respiratory syndrome coronavirus (SARS-CoV) nucleocapsid (N) protein-derived CTL epitope, N1 (QFKDNVILL), and the negative control (NC) peptide was a HLA-A2-restricted influenza A matrix peptide 58-66 (GILGFVFTL). All the peptide could refold with the HLA-A24 heavy chain and light chain. The results are representative of three independent experiments.