Table 2.
Predicted HLA-A*24-restricted peptides for TB epitopes.
| No. | Sequence | Position | Scorea | HLA-A24 bindingb |
|---|---|---|---|---|
| P1 | Q Q A L S S Q M G F | CFP10, 91–100 | 187.3 | High |
| P2 | Q W R G A A G T A A | CFP10, 42–51 | 316.5 | Intermediate |
| P3 | E L N N A L Q N L | ESAT-6, 64–72 | 1015.9 | Intermediate |
| P4 | A Y Q G V Q Q K W | ESAT-6, 50–58 | 609.6 | No |
| P5 | Q W N F A G I E A A | ESAT-6, 5–14 | 411.2 | Intermediate |
| P6 | Q Y S R A D E E Q Q | CFP10, 82–91 | 635.8 | No |
| P8 | V T S I H S L L D | ESAT-6, 22–30 | 631.4 | High |
| P9 | E M K T D A A T L | CFP10, 3–11 | 306.4 | No |
a
In the evaluation of HLA-A24-restricted peptide prediction, the predicted binding score in the computer algorithm is reported as the IC50 value. IC50 refers to the concentration of peptide required to inhibit 50% of reporter peptide-MHC binding. The source of the peptide binding prediction was a web site: www.immuneepitope.org. (see Ref. [15]).
b
The binding of peptides to the RMA-S/hβ2m-A24 cell line was demonstrated by an increase in the fluorescence index MFI (see Ref. [15]). Peptides with an MFI ratio >1.5 defines a high-affinity binding, and intermediate binders have an MFI ratio of 1.25–1.5. The other peptides showed no binding affinity.