Table 3.
Induction of P5-specific CTLs in PBMC cultures from TB-infected patients.
| IFN-γspot-forming unit | ||
|---|---|---|
| Group |
Ex vivo |
Cells cultured for 10 days in the presence of P5 peptide |
| Per 2 × 105 cells | Per 5 × 104 cells | |
| A24 TB patient 1 | ||
| Positive control (+) | 223 ± 77 | 583 ± 151 |
| Negative control (−) | 3 ± 3 | 11 ± 9 |
| Sample | 22 ± 20 | 213 ± 54 |
| A24 TB patient 2 | ||
| Positive control (+) | 226 ± 37 | 639 ± 67 |
| Negative control (−) | 8 ± 2 | 6 ± 4 |
| Sample | 36 ± 9 | 193 ± 48 |
| A24 healthy donor | ||
| Positive control (+) | 233 ± 42 | 548 ± 80 |
| Negative control (−) | 7 ± 4 | 8 ± 6 |
| Sample | 7 ± 5 | 48 ± 24 |
| A2 TB patient | ||
| Positive control (+) | 179 ± 35 | 519 ± 29 |
| Negative control (−) | 5 ± 4 | 6 ± 2 |
| Sample | 4 ± 4 | 58 ± 8a |
a
Mean ± SD from three independent experiments. Values shown are the mean number of IFN-γ+ cells, based on duplicated wells.