Fig. 4.

Overexpression of canine STING inhibits the replication of CIV H3N2. (A, B) MDCK cells were transfected with different doses of Flag-STING or empty vector. 24 h after transfection, cells pre-inoculated in 12 well plate were infected with 1000 TCID₅₀ of CIV H3N2. The RNA levels of viral M gene (A) and the expression of viral NP gene (B) were determined by qPCR and Western blotting, respectively, 24 h after infection. * represent differences between experimental and control groups (p < 0.05). (C, D) A549 cells were transfected with different doses of Flag-STING or empty vector. 24 h after transfection, cells pre-inoculated in 12 well plate were infected with 10000 TCID₅₀ of CIV H3N2. The RNA levels of viral M gene (C) and the expression of viral NP gene (D) were determined by qPCR and Western blotting, respectively, 24 h after infection.