Fig. 6.

Inhibition of genomic RV RNA synthesis by NTZ. HUVEC monolayers were infected with RV-Dz (A) or RA27/3 (B) at an MOI of 5 and then exposed to 2.5 μg/ml NTZ or vehicle for 48 h (A and B) Positive or negative strand RV RNA (red) and mRNA for GAPDH and β-actin (green) were detected by RNA-FISH. Nuclei were stained with DAPI. (C) Total fluorescence of each of six randomly picked macroscopic fields per each condition was determined by using ImageJ. Data are presented as a mean ± SD of fluorescence intensity in arbitrary units (AU) per a field. (For interpretation of the references to colour in this figure legend, the reader is referred to the web version of this article.)