Table 2.
The clones were selected as the target genes and a control gene.
| Library ID | Ratio of Cy5/Cy3-intensities | FCV §1 | Homologous genes | Accession No. | Identity §2 (%) | E value §3 |
|---|---|---|---|---|---|---|
| F123 | 1.1015 | <0.5 | CSH2* | NM_020991.3 | 688/694 (99%) | 0.0 |
| R5 | 7.6808 | >2 | EIF4G2* | NM_001042599.2 | 1001/1043 (96%) | 0.0 |
| F12 | 0.6321 | <0.5 | PCBD2* | NT_034772.6 | 569/590 (96%) | 0.0 |
| F14 | 1.4166 | <0.5 | PSG4* | NM_213633.1 | 526/536 (98%) | 0.0 |
| F11 | 5.8078 | >2 | TTN * | NM_133437.3 | 768/781 (98%) | 0.0 |
| F110 | 3.0834 | >0.5, <2 | ESRRG ¤ | NG_029784.1 | 740/744 (99%) | 0.0 |
§1 FCV: fold change value. In the current study, the two-fold average ratio of Cy5/Cy3 intensities was 5.740, and the 0.5-fold average ratio of Cy5/Cy3 intensities was 1.4349. Both are the thresholds for determining whether gene differential expression is statistically significant. §2Identities: the extent to which two nucleotide sequences have the same residues at the same positions in an alignment, often expressed as a percentage [36]. §3 E value: the Expectation value or Expect value represents the number of different alignments with scores equivalent to or better than S that is expected to occur in a database search by chance. The lower the E value, the more significant the score and the alignment [36]. * The target genes. ¤The control gene.