Table 1.
Accession numbers and oligonucleotides used for reverse transcription and amplification of cDNA
| HCoV | Accession number, position | Primer (5′→3′) | Cloning site |
|---|---|---|---|
| 229E | DQ243939a | GAACGAACATATGGCTACAGTCAAATGGG | NdeI |
| (VR-740) | GTGGATCCTTTAGTTTAC | BamHI | |
| OC43 | AY585228b | AAATTTTACATATGTCTTTTACTCCTGG | NdeI |
| (VR-759) | 29079–30425c | GGTGAATTCTCTTATATTTCT | EcoRI |
| NL63 | AY567487b | TAAACTAAACCATATGGCTAGTG | NdeI |
| 26133–27266c | TGGAATTCCAAAACAATTTAATGC | EcoRI | |
| SARS (FRA) | AY310120b | ACAAATTCATATGTCTGATAATGG | NdeI |
| 28120–29388c | GGGGATCCTGAGTGTTTATGCC | BamHI | |
| HKU1 (Caen) | DQ778921a | ATCTACCCGCTTAGTATGTCTTATAC | FauI |
| ATTAGAATTCATTCTCAATTAAG | EcoRI |
Boldface letters in primer sequences indicate restriction endonuclease cleavage sites.
a
Accession numbers for nucleocapsid genes.
b
Accession numbers for full genome sequences.
c
Nucleotide positions of corresponding nucleocapsid genes.