Fig. 4.

Inhibition of bovine S100A9 by small interfering RNA (siRNA)-enhanced virus replication. The S100 A9 knock down or control MDBK cells were infected with ncpBVDV2a 1373 with MOI of 1. The supernatant was collected at 12 h, 24 h, 36 h or 60 h post infection and analyzed for BVDV viral titer. There was a significant increase in viral production by 1.5 log10 at 24 h (p < 0.05) in S100A9 knocked out cells while at 36 h in S100A9 knock out cells also increased (0.5 log¹⁰) virus production compared to scrambled siRNA-transfected cells. Error bars are SEM.