Table 2.

The result of optimization of concentration of Mg²⁺ and dNTP.

Concentration of MgCl2 (mM)	Concentration of dNTP (mM)	Meana of Ct value	SDa of Ct value
4	1.2	23.40	1.09
4	1.4	22.38	0.65
4	1.6	25.25	0.16
4	1.8	28.51	1.38
6	1.2	26.63	0.36
6	1.4	23.06	0.74
6	1.6	23.31	0.04
6	1.8	27.31	0.59
8	1.2	31.29	0.59
8	1.4	29.02	0.38
8	1.6	25.90	0.32
8	1.8	25.09	0.28
10	1.2	33.81	1.12
10	1.4	29.86	0.35
10	1.6	28.23	0.08
10	1.8	27.49	0.17

^aMean and SD were produced from the repeats of RT-LAMP assay in triplicate.