Table 2.
Frameshifting efficiencies of wild type and mutant RNA signals from CYDV-RPV or MMTV in E. coli and yeast S. cerevisiae
|
E. coli |
S. cerevisiae |
|||
|---|---|---|---|---|
| Construct | Luciferase activity (RLU×10−5) | FS efficiency (%) | Luciferase activity (RLU×10−3) | FS efficiency (%) |
| CYDV: 0 | 7530±200 | (100) | 8030±250 | (100) |
| Wild type | 150±30 | 2.0±0.5 | 120±20 | 1.5±0.3 |
| Stem 1 | 140±20 | 1.9±0.4 | 30±3 | 0.4±0.1 |
| Stem 2 | 155±20 | 2.1±0.3 | 29±3 | 0.4±0.1 |
| Del enhancer | 170±30 | 2.3±0.4 | 15±1 | 0.2±0.05 |
| Shift site | 6±3 | 0.08±0.02 | 4±0.5 | 0.05±0.02 |
| Spacer (−3) | 120±5 | 1.6±0.1 | 10±2 | 0.1±0.03 |
| Spacer (−6) | 110±4 | 1.5±0.1 | 8±2 | 0.1±0.03 |
| Spacer (+6) | 110±5 | 1.5±0.1 | 9±3 | 0.1±0.04 |
| MMTV: 0 | 7600±300 | (100) | 8100±300 | (100) |
| MMTV: −1 | 530±70 | 7±1 | 490±50 | 6±1 |
| U13C | 520±60 | 7±1 | 480±50 | 6±1 |
| APK | 520±60 | 7±1 | 160±30 | 2±0.6 |
| ΔA14U13C | 450±50 | 6±1 | 80±3 | 1±0.5 |
Cells were normalized and frameshifting (FS) efficiencies were calculated as the ratio of luciferase activity between the zero frame construct and the −1 frame construct. Data shown here are averages of eight independent measurements.