Fig. 7.

Expression of GP73 protein in normal human tissues. Tissue- and cell-specific GP73 expression was studied in normal human autopsy tissues and in the liver of a patient with GCH by indirect immunohistochemistry, as noted in Section 2. GP73 immunoreactivity is represented by the red signal, with a blue background provided by the hematoxylin counterstain. (A) Colon, (B) lung, (C) kidney, (D) prostate, (E) normal liver, (F) liver with GCH (original magnification ×400). GP73 is predominantly expressed in cells of the epithelial lineage (indicated by arrows), including colonic enterocytes (A), bronchial epithelial cells (B), proximal and distal tubular epithelial cells (C), and prostate epithelial cells (D). In normal liver, GP73 is expressed in biliary epithelial cells (arrow) and in occasional hepatocytes (arrowhead) (E). In GCH, strong staining is present in heptocyte-derived multinucleated syncitia (arrow).