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. 2015 Aug 31;29(6):408–413. doi: 10.1016/j.mcp.2015.08.006

Table 1.

Primers used to perform duplex real time PCR assays.

Viruses Gene Primer sequences Annealing Amplicon size Ref.
Influenza A M PF:TCAGGCCCCCTCAAAGCC
PR:GGGCACGGTGAGCGTRAA
55 °C 158 This study
Influenza B M PF:TAGCAGAAGGCCATGAAAGCTC
PR:CGTTCCTAGTTTTACTTGCATTGAAT
55 °C 94 [20]
PIV1 and 3 L PF:GAGACTCTGAGCTGTTTTTTAAC
PR:GCTGTACTTTCAAATCTCCA
55 °C 71 This study
PIV2 L PF:TGCATGTTTTATAACTACTGATCTTGCTAA
PR:GTTCGAGCAAAATGGATTATGGT
55 °C 77 [21]
PIV4 L PF:CGCTAAATGAGCCAAGAAGG
PR:GACCCATGAAATCGAGTGCT
55 °C 182 This study
CoVI Polymerase PF:CAACGTATGTGTGCTATAGGC
PR:GTATTAACTATTTCAGCAGGAC
55 °C 74 This study
CoVII Polymerase PF:TGGTGGCTGGGATGATATGT
PR:GGCATAGCACGATCACACTTCAA
55 °C 96 [22]
hRV/EV 5′UTR PF:AGTCCTCCGGCCCCTGAA
PR:GAAACACGGCACCCCAAAGT
55 °C 120 [23]
hRSV L PF:AATACAGCCAAATCTAACCAACTTTA
PR:GCCAAGGAAGCATGCAATAAA
55 °C 94 [24]
hMPV F PF:GAGAGCTGAAAGAATTTGTGAGC
PR:GGTCCAATGATATTGCTGGTGTTA
55 °C 174 This study
hAdV Exon 6 PF:CCCITTYAACCACCG
PR:ACATCCTTBCKGAAGTTCCA
55 °C 167 [25]
HBoV NS PF:CTTGGGCGGGACAGAATGC
PR:AACAGAATTGCCACCAACAACC
55 °C 120 [26]