Table 1.
Primers used to perform duplex real time PCR assays.
| Viruses | Gene | Primer sequences | Annealing | Amplicon size | Ref. |
|---|---|---|---|---|---|
| Influenza A | M | PF:TCAGGCCCCCTCAAAGCC PR:GGGCACGGTGAGCGTRAA |
55 °C | 158 | This study |
| Influenza B | M | PF:TAGCAGAAGGCCATGAAAGCTC PR:CGTTCCTAGTTTTACTTGCATTGAAT |
55 °C | 94 | [20] |
| PIV1 and 3 | L | PF:GAGACTCTGAGCTGTTTTTTAAC PR:GCTGTACTTTCAAATCTCCA |
55 °C | 71 | This study |
| PIV2 | L | PF:TGCATGTTTTATAACTACTGATCTTGCTAA PR:GTTCGAGCAAAATGGATTATGGT |
55 °C | 77 | [21] |
| PIV4 | L | PF:CGCTAAATGAGCCAAGAAGG PR:GACCCATGAAATCGAGTGCT |
55 °C | 182 | This study |
| CoVI | Polymerase | PF:CAACGTATGTGTGCTATAGGC PR:GTATTAACTATTTCAGCAGGAC |
55 °C | 74 | This study |
| CoVII | Polymerase | PF:TGGTGGCTGGGATGATATGT PR:GGCATAGCACGATCACACTTCAA |
55 °C | 96 | [22] |
| hRV/EV | 5′UTR | PF:AGTCCTCCGGCCCCTGAA PR:GAAACACGGCACCCCAAAGT |
55 °C | 120 | [23] |
| hRSV | L | PF:AATACAGCCAAATCTAACCAACTTTA PR:GCCAAGGAAGCATGCAATAAA |
55 °C | 94 | [24] |
| hMPV | F | PF:GAGAGCTGAAAGAATTTGTGAGC PR:GGTCCAATGATATTGCTGGTGTTA |
55 °C | 174 | This study |
| hAdV | Exon 6 | PF:CCCITTYAACCACCG PR:ACATCCTTBCKGAAGTTCCA |
55 °C | 167 | [25] |
| HBoV | NS | PF:CTTGGGCGGGACAGAATGC PR:AACAGAATTGCCACCAACAACC |
55 °C | 120 | [26] |