Table 2.
Primers used to perform sequencing reaction of positive samples.
| Viruses | Gene | Primer sequences | Annealing | Amplicon size | Ref. |
|---|---|---|---|---|---|
| Influenza A | M | PF:GAGTCTTCTAACMGAGGTCGAAACGTA PR:GCTGCCTGTTCACTCGATCC |
55 °C | 597 | This study |
| Influenza B | M | PF:CACTGTTGGTTCGGTGGGAA PR:ACAAAGCACAGAGCGTTCCT |
55 °C | 367 | This study |
| PIV2 | L | PF:TCTCGCAAATCATGCAGGTACT PR:GCCTTCAATACCTCCCTTGGA |
55 °C | 496 | This study |
| CoVI | polymerase | PF:GCYCAYGCTGCTGTTGATTC PR:ACTRGARCCATTGTCWACCTG |
55 °C | 534 | This study |
| hRV/EV | 5′UTR | PF:GCACTTCTGTTTCCCCG PR:GAAACACGGCACCCCAAAGT |
55 °C | 390 | [23] |
| hRSV | L | PF:TAAGRATTGCTAATTCWGAATTAGA PR:TMCCWGCTCCTTCACCTATGA |
55 °C | 501 | This study |
| hMPV | F | PF:AACCATMCGRCTTGAGAGTG PR:GCTYCCGTAGACCCCTATCAG |
55 °C | 418 | This study |
| hAdV | Exon 6 | PF:CAACACCTAYGASTACATGAA PR:KATGGGGTARAGCATGTT |
55 °C | 474 | [25] |