Table 5.
Intra-assay and inter-assay variability of the results of the 6 duplex PCR assays performed with the positive controls (mean of the melting temperature of each amplicon ± standard deviation).
| Target | Intra-assay variability | Inter-assay variability |
|---|---|---|
| Influenza A | 82.65 ± 0.04 | 82.78 ± 0.33 |
| Influenza B | 78.18 ± 0.07 | 78.08 ± 0.09 |
| PIV2 | 74.10 ± 0.04 | 74.22 ± 0.40 |
| hMPV | 80.50 ± 0.05 | 80.10 ± 0.50 |
| CoVI 229E | 76.40 ± 0.12 | 76.35 ± 0.37 |
| CoVI NL63 | 75.70 ± 0.07 | 75.83 ± 0.06 |
| EV | 85.88 ± 0.14 | 85.90 ± 0.28 |
| hRV | 84.35 ± 0.12 | 84.28 ± 0.25 |
| PIV1 and 3 | 73.05 ± 0.05 | 73.02 ± 0.20 |
| hRSV | 76.88 ± 0.12 | 76.93 ± 0.62 |
| CoVII OC43 | 77.5 ± 0.02 | 77.2 ± 0.2 |
| PIV4 | 80.05 ± 0.12 | 80.02 ± 0.50 |
| hAdV | 83.98 ± 0.02 | 84.07 ± 0.25 |
| HBoV | 77.7 ± 0.2 | 78.02 ± 0.33 |