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. 2017 Aug 12;99:443–449. doi: 10.1016/j.bios.2017.08.027

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© 2017 Elsevier B.V. All rights reserved.

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Fig. 1 — Schematic diagram of rapid pathogen diagnostic system using a single-tube approach. (a) In situ sample processing involves pathogen enrichment by APTES-modified DE (diagram) within 40 min and DNA extraction by DMS (dimethyl suberimidate)-assisted APTES-DE (diagram) within 20 min. The ISAD biosensor is then used to detect pathogen obtained from the in situ sample processing within 20 min (b) Pathogen enriched via electrostatic interaction. (c) DNA extraction by DMS (dimethyl suberimidate)-assisted APTES-DE. This novel system can be used for the rapid, simple and sensitive diagnosis of pathogens.