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. 2017 Aug 12;99:443–449. doi: 10.1016/j.bios.2017.08.027

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© 2017 Elsevier B.V. All rights reserved.

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Fig. 2 — Characterization of the pathogen enrichment process using APTES-DE. (a) Experimental pathogen enrichment procedure. (b–c) Optimized protocols for enrichment were demonstrated using qRT-PCR with serially diluted concentrations of DE (b) and at varying incubation times (c). (d) Parallel experiments comparing the performances of the APTES-DE assay and a commercial kit with a series of cell concentrations; inset, linear relationship of both approaches between cycle threshold (Ct) and concentrations of pathogen. Bars extended to a negative control (NTC) line here means no amplification signals.