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. 2017 Aug 12;99:443–449. doi: 10.1016/j.bios.2017.08.027

Fig. 3.

Fig. 3

Characterization of the DNA extraction process using APTES-DE. (a) Experimental DNA extraction procedure. (b–c) Optimized protocols for DNA extraction were demonstrated using qRT-PCR with serially diluted concentrations of DE (b) and at varying incubation times (c). (d) Performance of the DNA extraction assay using APTES-DE with a series of cell concentrations 1–107 cells/ml); inset, linear relationship of the cycle threshold (Ct) as a function of the serial cell concentration. Bars extended to a negative control (NTC) line here means no amplification signals.