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. 2014 Mar 19;185:23–31. doi: 10.1016/j.virusres.2014.03.012

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Crown copyright © 2014 Published by Elsevier B.V. All rights reserved.

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Fig. 6 — H5N1 and IL6 appear co-localized in chicken lung and liver tissues as determined by immunohistochemistry. Left hand panel shows H5N1 infected chicken (94) lung tissue, right hand panel shows H5N1 infected chicken (76) liver tissue. (A) Lung tissue with blood vessels and lesions in parenchyma (hematoxylin and eosin). (B) Lung tissue showing H5N1 viral antigen staining in blood vessel endothelium and wall and within the lung parenchyma. (C) Lung tissue with IHC staining for IL6 within blood vessel walls and throughout the parenchyma. (D) Liver tissue with arrow identifying necrotic lesion (hematoxylin and eosin). (E) Liver tissue showing same lesion with IHC for H5N1 antigen staining throughout sinusoidal endothelial cells and necrotic hepatocytes. (F) Liver tissue showing same lesion with IHC for IL6 staining throughout necrotic cells (arrow). All scale bars = 50 μm.