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. 2006 Nov 10;366(1):67–81. doi: 10.1016/j.jmb.2006.11.011

Figure 3.

Figure 3

Expression of HIV-1 Tat in a microglial cell line promotes survival in response to apoptotic stimuli. (a) Control CHME5 subline cells (white bars, pCDNA3.1) and those expressing HIV-1 Tat (grey bars, pTat101) were either left untreated, or treated with LPS (10 or 100 μg/ml) plus 10 μg/ml of cycloheximide (CHX) or CHX alone for 6 h. The cells were harvested and viability was assessed by trypan blue exclusion (shown as a percentage of cell death). The data represent the mean ± SEM derived from three independent experiments. (b) Representative results from the cytotoxicity assay. Cells were treated with 10 μg/ml of LPS + CHX for 24 h before performing the cytotoxicity assay. Live cells are represented by fluorescent green staining (intracellular esterase activity) while dead cells appear fluorescent red (ethidium homodimer binding). The fields shown represent merged images. Three independent CHME5 subline clones were tested in this study.