Figure 7.

HIV-1 infection protects primary human macrophage from cell death. Primary human macrophage were either infected with 1.1 × 10⁷ pg M-tropic HIV-1 BAL ((a) and (c)) or transduced with pseudotyped HIV-GFP vector ((b) and (d)) at an MOI of 40 for two days prior to treatment. Heat-inactivated virus or vector was used as a control. Following 24 h exposure to LPS/CHX or SNP, cells were analyzed for viability using the cytotoxicity assay. LPS was used at a concentration of 15 μg/ml (BAL infections) or 25 μg/ml (viral transductions) with a constant CHX concentration of 10 μg/ml while SNP was used at 1 mM. Representative bright fields (BF) and merged fields (green + red) are shown. Green cells represent either viable cells ((a) and (c)) or cells expressing GFP ((b) and (d)). In (a)–(d), red cells represent dead cells stained with ethidium homodimer.