Fig. 8.

CsA treatment blocks AIF nuclear translocation. (A) Immunofluorescent detection of AIF in the presence of CsA. Vero cells were preincubated with CsA (5 μM) for 1 h and were mock-infected or infected with PEDV (MOI of 0.1). At 24 hpi and 48 hpi, cells were labeled with MitoTracker Red CMXRos (red), fixed, and incubated with an anti-AIF antibody (green). AIF mitochondrial colocalization is represented by merged AIF and the mitochondrial marker (yellow). (B) Western blot analysis of AIF in the presence of CsA. Each mitochondrial and nuclear fraction was prepared under the indicated conditions and subjected to western blotting with an antibody against AIF (top panel), VDAC as a mitochondrial protein marker (middle panel), or Sp1 as a nuclear protein marker (bottom panel). Lane M, mock-infected.