Fig. 10.

N-PhMI treatment hampers AIF nuclear translocation. (A) Immunofluorescent detection of AIF in the presence of N-PhMI. Vero cells were pretreated with N-PhMI CsA for 1 h and were mock-infected or infected with PEDV (MOI of 0.1). At 24 hpi and 48 hpi, cells were labeled with MitoTracker Red CMXRos (red), fixed, and incubated with an anti-AIF antibody (green). AIF mitochondrial accumulation is indicated by merged AIF and the mitochondrial marker (yellow). (B) Western blot analysis of AIF in the presence of N-PhMI. Each mitochondrial and nuclear fraction was prepared under the indicated conditions and subjected to western blotting with an antibody against AIF (top panel), VDAC as a mitochondrial protein marker (middle panel), or Sp1 as a nuclear protein marker (bottom panel). Lane M, mock-infected.