Table 1.

Frameshift efficiency for different mutants of group O of HIV-1

		Frameshift efficiency (%)
Construct	Description	In vitro	In cultured cells
Frameshift efficiency for regions of different length of group O
pHIV/O-87-LUC	Long frameshift region	10.2 (100)	4.7 (100)
pHIV/O-60-LUC	Short frameshift region	3.3 (32)	1.9 (40)
Frameshift efficiency for mutants of group O
pHIV/O-k/o-LUC	Altered slippery sequence	1.0 (10)	0.2 (4)
pHIV/O-1.2-LUC	Substitution (3′ strand of stem 1)	3.4 (33)	0.6 (13)
pHIV/O-1.12-LUC	Substitution (stem 1 re-formed)	10.0 (98)	4.5 (96)
pHIV/O-DSL-LUC	Deletion (minus stem 1 and its capping loop)	2.3 (23)	0.4 (9)
Frameshift efficiency for mutations impairing or re-forming the pseudoknot
pHIV/O-2.1-LUC	Substitution (loop capping stem 1)	4.4 (43)	1.6 (34)
pHIV/O-2.2-LUC	Substitution (downstream segment)	3.9 (38)	2.1 (45)
pHIV/O-2.12-LUC	Compensatory (pseudoknot re-formed)	10.3 (101)	3.8 (81)
pHIV/O-ANT70-LUC	Long frameshift region for subtype ANT70 of group O	4.0 (39)	2.2 (47)

All pHIV/O-LUC constructs contain the HIV-1 gag/pol frameshift region of group O MVP5180 (except for construct pHIV/O-ANT70-LUC). Mutants of the group O frameshift region are further identified by a short description recalling their characteristics (see details in Figure 3, Figure 4). For each (−1) construct, an in-frame (0) control was made to monitor the frameshift efficiency. The numbers between brackets represent the frameshift efficiency of each construct relative to pHIV/O-87-LUC, which is arbitrarily set at 100%. Results are the means of five to six independent experiments. Standard error on the mean was inferior or equal to 10%.