Fig. 8.

Inhibition of ERK phosphorylation during TC-83 infection improves survival of U87MG cells. (A) U87MG cells were seeded in a 12-well plate and infected with TC-83 (MOI:1). Treatment of Ag-126 was included at 10 μM. DMSO was maintained as a negative control. Images were taken at 24 hpi, with controls being Ag-126 treatment alone and untreated uninfected cells. Rows A–D, E–H and I–L represent biological triplicate experiments. The conditions in each panel for every column are indicated in the table above the images. (B and C) U87MG cells were seeded in white walled 96 well plates and infected with TC-83, with or without Ag-126 treatments (10 μM). At 24 hpi (B) and 48 hpi (C), biological triplicate wells were subjected to Cell-Titer-Glo assay. X-axis indicates the amount of cell survival and Y-axis indicates the drug treatment conditions. All experiments were repeated three independent times.