Table 1.
Primers used in the present study
| Primer | Sequencea | Viral genome positionb |
|---|---|---|
| For fragment A | ||
| P1F | 5′GCATATAAGCTTTAATACGACTCACTATAGGTGCCTCGGCAT TTGTATTGTCAGG | 24–47 |
| P1146R | 5′GTGTCAGTCGACGTAGATGTACAACGATAGGCCCATC | 1155–1174 |
| T7P+1 | 5′ATATAAGCTTCGGACCGTAATACGACTCACTATAGGATGACGTATAGGTGTTGGCTCTATGCCATGACATTTGTATTG | 1–42 |
| For fragment B | ||
| P1145F | 5′GTGTCAGATATCTGTACAGTACTTCTCTG | 1169–1185 |
| P6154R | 5′GTGTCAGATATCAGCCCATATGTCTTTAATTATGTGGC | 6157–6178 |
| For fragment 5C | ||
| P5706F | 5′ATATAGATATCGCACATGTCCTTACGGG | 5730–5746 |
| P9791R | 5′ATATAGATATCCATCTAGAGGGCTTGTGC | 9798–9815 |
| For fragment 3C | ||
| P9783F | 5′ATATAGGATCCCTCTAGATGAGGTGTTG | 9805–9823 |
| P13625R | 5′ATATAGGATCCCTTGACATGTTGGACGTAGC | 13640–13660 |
| For fragment D | ||
| P12687F | 5′GTGTCAAAGCTTGTACATTCCTCCATATTTTCCTCC | 12710–12734 |
| P15393RpolyA | 5′GTGTCAGTCGACGCGTTTTTTTTTTTTTTTTTTTTTTTTTTTT TTTTTTTTTTTTTAATTTCGGCCGCATGGTTCTCGC | 15391–15454 |
| For genetic Tag | ||
| P24F | 5′TGCCTCGGCATTTGTATTGTC | 24–44 |
| P1763R | 5′CACATTCAAGGGGGAGCAGAGAAG | 1741–1764 |
a
T7 RNA polymerase promoter sequence is italicized. Underlined sequences represent restriction enzyme recognition sites utilized in full-length cDNA assembly. PRRSV-specific sequences are in bold-face type.
b
Nucleotide position within the NVSL#97-7895 PRRSV isolates consensus full-length genome.