Table 1.
Panel of antibodies used in this study to characterize astrocytes, T and B lymphocytes, macrophages, and matrix metalloproteinases 2 and 9 in the in the cerebellum of dogs with subacute distemper leukoencephalitis.
| Antibody | Specificity | Dilution | Pre-treatment | Source |
|---|---|---|---|---|
| Polyclonal rabbit anti-human GFAP (glial fibrillary acidic protein) | Astrocytes | 1:500 | Trypsin 37 °C for 30 min | Sigma–Aldrich, G9269 |
| Polyclonal rabbit anti-human CD3 | T lymphocytes | 1:200 | Citrate pH 6.0 in steamer for 30 min | Dako, A0452 |
| Monoclonal mouse anti-human CD79α, clone HM57 | B lymphocytes | 1:50 | Tris–EDTA–Tween pH 9.0 in steamer for 30 min | Dako, M7051 |
| Monoclonal mouse anti-human Myeloid/Histiocyte antigen, clone MAC387 | Macrophages/microglial cells | 1:100 | Citrate pH 6.0 in steamer for 30 min | Dako, M0747 |
| Monoclonal mouse anti-MMP-2 | MMP-2 (active and pro-forms) | 1:100 | Citrate pH 6.0 in steamer for 30 min | Calbiochem, IM51 |
| Polyclonal rabbit anti-human MMP-9 | MMP-9 (active and pro-forms) | Ready-to-use | Tris–EDTA–Tween pH 9.0 in steamer for 30 min | NeoMarkers, RB-9234-R7 |