Fig. 1.

PHB2 interacts with CaMKIV through C-terminus in yeast cells. (A) CaMKIVca cDNA was cloned into pGBKT7 and different deletion fragments of PHB2 were separately cloned into pGADT7. The mutual interaction between CaMKIV and individual PHB2 variants were tested by yeast two-hybrid assays. Positive clones were checked by being streaked on synthetic dropout plates lacking leucine, tryptophan, histidine and adenine (SD/-his/-leu/-trp/-ade). Akt-pGBKT7 and pGBKT7 were used as PHB2-binding positive and negative control respectively. The numbers marked on the plate represent the position of amino acids in various truncated PHB2. (B) Schematic representation of yeast two-hybrid assays of (A). The black bar represents the CaMKIV-interacting region on PHB2. The plus and minus signs denote growth and no growth on SD/-his/-leu/-trp/-ade plates. (C). The expression levels of different PHB2 fragments in AH109 yeast cells were detected by immunoblotting.