Table 3.
Recoveries, matrix effects and stability of neochlorogenic acid, chlorogenic acid, cryptochlorogenic acid, 3,5-dicaffeoylquinic acid and 3,4-dicaffeoylquinic acid (n = 6).
| Compound | Concentration (ng ml−1) | Recovery |
Matrix effect |
Freeze-thaw cycles |
At −70 °C for 1 month |
Autosampler for 24 h |
|||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| Average (%) | RSD (%) | Average (%) | RSD (%) | Remain (%) | RSD (%) | Remain (%) | RSD (%) | Remain (%) | RSD (%) | ||
| Neochlorogenic acid | 1.50 | 40.09 | 12.4 | 97.9 | 6.2 | 106.8 | 6.7 | 102.6 | 8.3 | 91.4 | 13.8 |
| 20.0 | 40.6 | 10.4 | 110.2 | 1.3 | 92.9 | 13.4 | 107.3 | 14.1 | 108.2 | 14.1 | |
| 250 | 42.0 | 8.2 | 110.7 | 11.8 | 100.9 | 4.6 | 85.7 | 13.8 | 88.2 | 9.0 | |
| Chlorogenic acid | 1.00 | 59.9 | 11.5 | 101.9 | 8.7 | 99.2 | 7.5 | 108.7 | 11.0 | 98.5 | 9.9 |
| 25.0 | 67.7 | 12.7 | 113.3 | 5.5 | 110.6 | 8.5 | 114.9 | 10.5 | 111.8 | 11.8 | |
| 650 | 60.7 | 4.6 | 111.4 | 2.4 | 98.9 | 6.8 | 90.0 | 11.1 | 87.8 | 11.6 | |
| Cryptochlorogenic acid | 4.00 | 57.7 | 8.1 | 102.1 | 8.7 | 91.2 | 11.4 | 100.7 | 12.5 | 114.3 | 12.7 |
| 25.0 | 64.0 | 10.8 | 102.2 | 9.8 | 96.8 | 7.3 | 105.4 | 13.4 | 86.6 | 9.9 | |
| 160 | 58.7 | 4.1 | 97.1 | 14.3 | 105.4 | 10.1 | 104.4 | 9.6 | 99.3 | 6.1 | |
| 3,5-dicaffeoylquinic acid | 1.50 | 75.0 | 14.5 | 98.5 | 6.5 | 92.5 | 7.2 | 111.3 | 12.6 | 104.5 | 5.1 |
| 20.0 | 82.8 | 8.1 | 103.2 | 10.4 | 87.7 | 11.8 | 85.2 | 12.8 | 90.8 | 13.7 | |
| 250 | 77.3 | 11.7 | 113.6 | 1.0 | 88.4 | 8.6 | 93.3 | 10.8 | 94.4 | 10.2 | |
| 3,4-dicaffeoylquinic acid | 10.0 | 80.4 | 10.2 | 104.6 | 4.6 | 95.6 | 11.1 | 93.9 | 11.3 | 98.3 | 10.1 |
| 45.0 | 70.9 | 13.6 | 102.0 | 7.2 | 97.5 | 9.8 | 108.8 | 8.3 | 86.0 | 12.5 | |
| 220 | 89.6 | 9.6 | 112.6 | 9.6 | 108.4 | 9.0 | 86.1 | 11.23 | 86.86 | 11.2 | |