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. 2008 Dec 4;86(3):443–452. doi: 10.1016/j.rvsc.2008.10.011

Fig. 1.

Fig. 1

Agarose gel analysis of siRNA molecules generated using the T7 RNA polymerase. Each siRNA was separated on a 1% agarose gel/1× TAE and visualized by staining the gel with 0.5 μg/ml ethidium bromide. Lane designations: lane 1, 100 bp DNA ladder (GenScript Corp.); lane 2, pEGFP-N1-siRNA; lane 3, scrambled-siRNA; lane 4, VP1-siRNA517; lane 5, VP1-siRNA113; lane 6, VP1-siRNA616; lane 7, VP1-siRNA519; lane 8, VP1-siRNA401. Note: siRNA migrates more slowly than double-stranded DNA. Only those five efficacious siRNAs out of siRNAs candidates targeting the VP1 of FMDV are shown.