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Expression of pVP1-EGFP-N1 in HEK 293T cell line. (A) The pVP1-EGFP-N1 reporter constructs. The complete VP1 sequence of FMDV genome was inserted upstream of the EGFP gene. (B) pVP1-EGFP-N1 expressing VP1-EGFP in HEK 293T cells. Bright-field image (left) and the corresponding fluorescent-field image (right) were taken with fluorescence microscope (Olympus IX70, Japan) at 48 h after transient transfection.
