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. 2008 Dec 4;86(3):443–452. doi: 10.1016/j.rvsc.2008.10.011

Fig. 3.

Fig. 3

Screening of sequence-specific siRNAs inhibiting the expression of VP1-EGFP in HEK 293T cells. (A) Shown here is the bright-field image (left) and the relative fluorescent-field image (right) at 48 h after a cotransfection assay of all siRNAs with pVP1-EGFP-N1. (B) Flow cytometry analysis of VP1-EGFP expression at 48 h after cotransfection. The percentage of the cell population that exceeded the fluorescence intensity of the control cells (HEK 293T) and the mean fluorescence intensity of this population were calculated. (C) Relative GFP expression was measured with the corresponding data from flow cytometry. VP1-EGFP production in the absence of siRNA inhibitor was set at 100% (pVP1-EGFP-N1) for the negative control. (D) Relative GFP expression was measured after a cotransfection of all siRNAs with p3C-EGFP-N1 in HEK 293T cells. The mean values and standard deviations obtained from three independent cotransfections are shown. ∗P < 0.05 vs. scrambled-siRNA group.