Fig. 7.
Influences of single nucleotide substitution within or near the siRNA target region on RNAi resistance. (A) HEK 293T cells were cotransfected with pVP1-EGFP-N1-410G or pVP1-EGFP-N1-410A with VP1-siRNA401. (B) HEK 293T cells were cotransfected with pVP1-EGFP-N1-607C or pVP1-EGFP-N1-607T with VP1-siRNA616. Relative GFP expression was measured by Flow cytometry analysis at 48 h after cotransfection. The mean values and standard deviations obtained from three independent cotransfections are shown.