Table 1.

siRNAs molecules targeting VP1 of FMDV and their template oligonucleotides.

siRNAs	siRNA sequences	Template oligonucleotide structuresb
VP1-siRNA517	5′-GUGACUGAACUGCUUUACCUU-3′	5′-AAGGTAAAGCAGTTCAGTCACTATAGTGAGTCGTATTAGGATCC-3′
	3′-UUCACUGACUUGACGAAAUGG-5′	5′-AAGTGACTGAACTGCTTTACCTATAGTGAGTCGTATTAGGATCC-3′
VP1-siRNA113	5′-GAUUUGUGAAAGUAACACCUU-3′	5′-AAGGTGTTACTTTCACAAATCTATAGTGAGTCGTATTAGGATCC-3′
	3′-UUCUAAACACUUUCAUUGUGG-5′	5′-AAGATTTGTGAAAGTAACACCTATAGTGAGTCGTATTAGGATCC-3′
VP1-siRNA616	5′-GUGGCGCCUGUGAAACAGCUU-3′	5′-AAGCTGTTTCACAGGCGCCACTATAGTGAGTCGTATTAGGATCC-3′
	3′-UUCACCGCGGACACUUUGUCG-5′	5′-AAGTGGCGCCTGTGAAACAGCTATAGTGAGTCGTATTAGGATCC-3′
VP1-siRNA519	5′-GACUGAACUGCUUUACCGCUU-3′	5′-AAGCGGTAAAGCAGTTCAGTCTATAGTGAGTCGTATTAGGATCC-3′
	3′-UUCUGACUUGACGAAAUGGCG-5′	5′-AAGACTGAACTGCTTTACCGCTATAGTGAGTCGTATTAGGATCC-3′
VP1-siRNA401	5′-GCAAGUAUGGCGAGAGCCCUU-3′	5′-AAGGGCTCTCGCCATACTTGCTATAGTGAGTCGTATTAGGATCC-3′
	3′-UUCGUUCAUACCGCUCUCGGG-5′	5′-AAGCAAGTATGGCGAGAGCCCTATAGTGAGTCGTATTAGGATCC-3′
pEGFP-N1-siRNA	5′-GCUGACCCUGAAGUUCAUCUU-3′	5′-AAGATGAACTTCAGGGTCAGCTATAGTGAGTCGTATTAGGATCC-3′
	3′-UUCGACUGGGACUUCAAGUAG-5′	5′-AAGCTGACCCTGAAGTTCATCTATAGTGAGTCGTATTAGGATCC-3′
Scrambled-siRNA	5′-GGUUCAUCGGCACUUCAACUU-3′	5′-AAGTTGAAGTGCCGATGAACCTATAGTGAGTCGTATTAGGATCC-3′
	3′-UUCCAAGUAGCCGUGAAGUUG-5′	5′-AAGGTTCATCGGCACTTCAACTATAGTGAGTCGTATTAGGATCC-3′
T7 RNA Pol oligoa		5′-GGATCCTAATACGACTCACTATAG-3′

^aT7 RNA Pol oligo: the T7 RNA polymerase promoter sequence and six extra nucleotides upstream of the minimal promoter sequence.

^bThe underlined regions of each template are the corresponding T7 promoter sequences.