Fig. 5.

Antigen-sparing effect of Advax adjuvant on JJAV-specific IgG responses. Female BALB/c mice, 6–8 week old (n = 5/group) were immunized 4 times i.m. at 2-week intervals with JJAV 2 μg alone or with Adva x 1 mg compared to a 5-fold higher dose of JJAV 10 μg alone, all in 50 μL total volume. Blood samples were collected 2 weeks after the last immunization and JJAV-specific IgG, IgG1 and IgG2a measured by ELISA (* p < 0.05, ** p < 0.01).