Fig. 6.

Transmission of DpRV-1 by D. pulchellus to A. assectella. RT-PCR of the 1.6 kb was performed on ds RNA extracted from washed (1) or unwashed (2) parasitized pupae, DpRV-1 artificially contaminated pupae, unwashed (3) or washed (4), unparasitized pupae (5), D. pulchellus eggs (7), and parasitized pupae at 1-day (6), 5-days (8) and 14-days (10) after parasitization, and D. pulchellus larvae 5 days after eggs were laid in the host (9). The+and – lanes correspond to positive and negative PCR controls.