Fig. 4.

Mucosally vaccinated and PRRSV challenged pig immune cells secreted increased recall Th1 and suppressed immunosuppressive cytokines. Experimental details were as described in legend to Fig. 1, and the data from only DPC 15 pigs is shown. (A) PBMC was restimulated in the presence of killed MN184 antigens and the IFN-γ-secreting cells were analyzed by the ELISPOT assay. Supernatants harvested from lung MNC and PBMC cultures restimulated using killed MN184 antigens or PRRSV recombinant matrix (M3′) protein were analyzed for cytokines: (B) IL-10; (C) IL-6 by ELISA. Each bar represents the average number of IFN-γ-secreting cell spots or amount of cytokines from three pigs ± SEM. Asterisk denotes a statistically significant difference (P < 0.05) between unvaccinated vs. vaccinated and PRRSV challenged pigs.