Figure 1.

(A) Differential expression of the RNF8 gene in the obstructive azoospermia (OA), nonobstructive azoospermia (NOA)+ (presence of sperm), and NOA-(absence of sperm) groups. ACTB was used as a housekeeping gene. Each reaction was performed in triplicate. Expression of RNF8 was upregulated in the NOA–group (2.27 ± 0.18) compared with the NOA+ (1.6 ± 0.23) and OA (1.3 ± 0.17) groups. The difference was significant (p= 0.001). (B) Receiver operating characteristic (ROC) curve of RNF8 expression in predicting sperm recovery outcomes.